High performance liquid chromatography:
Especially refers to a chromatographic separation and analysis method using liquid as the mobile phase. It uses a high-pressure pump, chemical
Bonded stationary phase high-efficiency separation column, high-sensitivity special detector, etc.
A liquid chromatography analysis method established by laboratory technology.
High pressure: 150-350 * 105Pa
High efficiency: more than 30,000 trays / meter
High sensitivity: 10-9g (ultraviolet detection), 10-11g (fluorescence detection)
The difference between liquid chromatograph and gas chromatograph:
1. Analysis of the difference between objects
GC: suitable for gasification, good thermal stability, and relatively high boiling point
Low samples; but for high boiling points, poor volatility,
Poor thermal stability, ionic and polymer samples
Products, especially undetectable for most biochemical samples
20% of organic matter
HPLC: suitable for samples that can be made into solutions after dissolution (including
Organic medium solution), not subject to sample volatility and
The limitation of thermal stability is difficult for large molecular weight
Biochemical samples with poor gasification and thermal stability and high scores
Both sub- and ionic samples can be detected
Widely used, accounting for 80% of organic matter
2. The difference between mobile phase differences
GC: the mobile phase is inert, and the gas component has no affinity with the mobile phase
Force, only interacts with the stationary phase.
HPLC: the mobile phase is a liquid, and there is an affinity between the mobile phase and the components
Force, can improve the selectivity of the column, improve the resolution, and improve the separation
Positive effect. And there are many types of mobile phases, and there is a wide range of choices.
Variable mobile phase polarity and pH also play a role in regulating separation, when
Use two or more liquids in different proportions as the mobile phase
Separation selectivity can also be increased.
3. Differences in operating conditions
GC: heating operation
HPLC: room temperature; high pressure (large liquid viscosity, small peak broadening)
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