What is a liquid chromatograph? A liquid chromatograph is an instrument that first separates the mixture and then analyzes it by using the difference in the distribution ratio between the liquid-solid or immiscible liquids. Today we mainly introduce the application and components of the liquid chromatograph, hoping to help users better apply the product.
Application of liquid chromatograph
Liquid chromatography requires only a sample to be made into a solution, independent of the volatility of the sample, a wide range of mobile phases, and a wide variety of stationary phases, thus separating thermally unstable and non-volatile, dissociated and non-volatile Dissociated and various molecular weight ranges of substances. In combination with the sample pretreatment technology, the high resolution and high sensitivity achieved by HPLC make it possible to separate and simultaneously measure substances of similar nature, which can separate trace components in complex phases. With the development of the stationary phase, it is possible to complete its separation HPLC under the condition of fully maintaining the activity of biochemical substances as the most promising method for solving the problem of biochemical analysis.
Because HPLC has high resolution, high sensitivity, fast speed, reusable column, easy collection of effluent components, it is widely used in various fields such as biochemistry, food analysis, medical research, environmental analysis, inorganic analysis, etc. . The combination of high performance liquid chromatography and structural instruments is an important development direction. Liquid chromatography-mass spectrometry technology has received widespread attention, such as the analysis of carbamate pesticides and polynuclear aromatic hydrocarbons; liquid chromatography-infrared spectroscopy has also been developed rapidly as measured by environmental pollution analysis of hydrocarbons in water, non-volatile in seawater Hydrocarbons have led to new developments in environmental pollution analysis.
Liquid chromatograph components
The system consists of a reservoir, a pump, an injector, a column, a detector, a recorder, and the like. The mobile phase in the reservoir is driven into the system by a high pressure pump. The sample solution enters the mobile phase through the injector and is loaded into the column (stationary phase) by the mobile phase, since each component in the sample solution has two phases. Different partition coefficients, when moving in two phases, after repeated adsorption-desorption distribution processes, the components have a large difference in the moving speed, and are separated into individual components and sequentially flow out from the column. When passing through the detector, the sample concentration is converted into an electrical signal and transmitted to the recorder. The data is printed in the form of a map. The high performance liquid chromatograph mainly has a sampling system, an infusion system, a separation system, a detection system, and a data processing system.
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