The SWATH â„¢ acquisition mode provides the world's scientific community with breakthrough technologies for proteomics research
Running the data-independent SWATH â„¢ acquisition mode on the TripleTOF â„¢ 5600 system enables comprehensive and complete protein quantification. The relevant results are published in Molecular and Cellular Proteomics.
The global life science analysis technology leader, AB SCIEX, in cooperation with the Zurich Federal Institute of Technology, has completed the development of the MS / MSALL SWATH â„¢ acquisition mode. The SWATH â„¢ acquisition mode is a mass spectrometry-based, used in proteomics The breakthrough technology of research can quantify all the proteins and peptides of a sample in a single analysis. This method was published in this year's "Molecular and Cellular Proteomics" (MCP).
Liquid chromatography tandem mass spectrometry (LC / MS / MS) is widely used in proteomics research, but at present this method cannot provide the speed, quantitative accuracy, specificity, sensitivity and repeatability required to make a complete quantitative proteome biomap . The SWATH â„¢ acquisition mode uses a data-independent MS / MS acquisition method for the first time, which can systematically generate a complete and highly specific picture of fragment ions. By using the target data analysis method, you can query any interest. Whether the protein is present and quantify it.
This new method relies on an advanced quadrupole time-of-flight hybridization mass spectrometer, AB SCIEX's TripleTOF 5600 system. The system combines high resolution, high quality accuracy and ultra high MS / MS spectrum acquisition rate, which means it is the only mass spectrometer that can effectively run the SWATH method. The SWATH â„¢ acquisition mode running on the TripleTOF 5600 system is an extension of AB SCIEX MS / MSALL technology. As part of the collaboration between AB SCIEX and the Ruedi Aebersold PhD group of the Federal Institute of Technology in Zurich, the technology will be available soon.
“The SWATH â„¢ acquisition mode allows us to explore multiple MS / MS data sets to a level that is impossible with traditional clustering or database methods,†Ruedi, Institute of Molecular Systems Biology, ETH Zurich, Switzerland Dr. Aebersold said, “By working with AB SCIEX, we have developed a breakthrough method running on TripleTOF â„¢ 5600 that can help proteomics researchers achieve a comprehensive quantitative analysis of the entire proteome.â€
This technology can generate highly specific fragment ion data for all precursor ions within the monitoring range. Unlike traditional acquisition methods, this technique does not rely on the detected mass of precursor ions to trigger MS / MS acquisition, but systematically breaks all the components in the sample into fragments through a fast-moving selection window.
For the obtained result data, using selective reaction monitoring or multiple reaction monitoring (SRM / MRM), for each peptide of interest, several high-resolution fragment ion chromatograms can be extracted to identify and quantify the target peptide . Combined with ion libraries in public resources such as MRMAtlas or generated from database search software such as ProteinPilotTM, researchers can repeatedly review quantitative data on peptides / proteins of interest. This technology can provide complete quantitative and qualitative results of samples, and can be retrospectively excavated through computer simulation based on new hypotheses developed.
references:
Targeted data extraction of the MS / MS spectra generated by data independent acquisition: a new concept for consistent and accurate proteome analysis
Ludovic C. Gillet, Pedro Navarro, Stephen Tate, Hannes Roest, Nathalie Selevsek, Lukas Reiter, Ron Bonner, and Ruedi Aebersold
Mol Cell Proteomics mcp.O111.016717. First Published on January 18, 2012, doi: 10.1074 / mcp.O111.016717
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