Indirect antigen competition method The difference between the indirect antigen competition method and the enzyme-labeled antigen competition method is that the enzyme-labeled antigen is not used, but the enzyme-labeled antibody is used for antigen determination. The general process is as follows: first, the purified antigen is adsorbed on the surface of the carrier to form an antigen-sensitized carrier, and the antigen-sensitized carrier is used in two detection systems, namely, a reference system and a system to be tested. Add the appropriate amount of the reference antibody and the antigen to be tested to the test plate, and add only the reference antibody to the parallel reference plate. After the reaction, the reference antibody in the reference plate fully binds to the solid-phase antigen, while the reference antibody does not bind to the solid-phase antigen due to the competition of the antigen under test. The free antigen-antibody complex is removed by washing, and the solid-phase antigen-antibody complex is retained. Then add enzyme-labeled anti-antibodies to the reference plate and the test plate respectively to form an enzyme-labeled immune complex. Finally, the substrate is added to produce a colored substance. Since the amount of enzyme-labeled anti-antibody bound in the reference plate is greater than that of the plate to be tested, the reaction color is dark, and the color difference between the reference plate and the plate to be tested indicates the amount of antigen to be tested. The indirect method of antigen competition due to the complex measurement process, so less in actual use.
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